Human HBeAg ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum and plasma

Human HBeAg ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human HBeAg. No significant cross-reactivity or interference between human HBeAg and analogues was observed.
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 20
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HBeAg ELISA Kit (Colorimetric)

• HBeAg

Background

This assay employs the qualitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBeAb. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated HBeAb. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HBeAg bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.