Mouse HBeAg Antibody ELISA Kit (Colorimetric) Summary
| Description |
Assay Length: 1-5 hours |
| Specificity |
This assay has high sensitivity and excellent specificity for detection of mouse HBeAb. No significant cross-reactivity or interference between mouse HBeAb and analogues was observed. |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
%CV < 20 |
| Intra-Assay |
%CV < 15 |
| Sample Volume |
50 - 100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Strip (For 96 wells)
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (20 x concentrate)
|
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HBeAg. Samples are pipetted into the wells with HBeAb conjugated Horseradish Peroxidase (HRP). Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of mouse HBeAb bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for HBeAg Antibody ELISA Kit (NBP2-60031). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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