H-2Dk Antibody (15-5-5)

Product Discontinued

H-2Dk Antibody (15-5-5) Summary

• Immunogen: This antibody was generated by immunizing host with C3H mouse splenocytes
• Localization: Nucleated cells of the H-2D^k haplotype
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse k haplotype, Cross-reacts with H-2K d and H-2 f haplotypes. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for H-2Dk Antibody (15-5-5)

• H2Dk
• MHC class I

Background

The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.