H-2Dk Antibody (15-5-5)

Images

 
Flow Cytometry: H-2Dk Antibody (15-5-5) [NBP1-48977] - C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, ? isotype control (open histogram), followed by ...read more

Product Details

Summary
Product Discontinued
View other related H-2Dk Primary Antibodies

Order Details


    • Catalog Number
      NBP1-48977
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

H-2Dk Antibody (15-5-5) Summary

Immunogen
This antibody was generated by immunizing host with C3H mouse splenocytes
Localization
Nucleated cells of the H-2Dk haplotype
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse k haplotype, Cross-reacts with H-2K d and H-2 f haplotypes. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for H-2Dk Antibody (15-5-5)

  • H2Dk
  • MHC class I

Background

The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for H-2Dk Antibody (NBP1-48977) (0)

There are no publications for H-2Dk Antibody (NBP1-48977).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for H-2Dk Antibody (NBP1-48977) (0)

There are no reviews for H-2Dk Antibody (NBP1-48977). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for H-2Dk Antibody (NBP1-48977) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our H-2Dk Antibody (15-5-5) and receive a gift card or discount.