H-2Dk Antibody (15-5-5)


Flow Cytometry: H-2Dk Antibody (15-5-5) [NBP1-48977] - C3H/He mouse splenocytes were stained with purified H-2Dk (clone 15-5-5) (filled histogram) or mouse IgG2a, ? isotype control (open histogram), followed by ...read more

Product Details

Reactivity MuSpecies Glossary
Applications Flow, IP
0.5 mg/ml

Order Details

H-2Dk Antibody (15-5-5) Summary

This antibody was generated by immunizing host with C3H mouse splenocytes
Nucleated cells of the H-2Dk haplotype
IgG2a Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.2)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse k haplotype, Cross-reacts with H-2K d and H-2 f haplotypes. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Alternate Names for H-2Dk Antibody (15-5-5)

  • H2Dk
  • MHC class I


The 15-5-5 antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 15-5-5 antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Additional H-2Dk Antibody (15-5-5) Products

H-2Dk NBP1-48977

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