H-2Dd Antibody (34-2-12) [DyLight 405LS]

Product Discontinued

H-2Dd Antibody (34-2-12) [DyLight 405LS] Summary

• Immunogen: (C57BL/6 x DBA/2)F1 mouse splenocytes
• Specificity: H-2Dd (34-2-12)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen

Reactivity Notes

Mouse d haplotype, Cross-reacts with the C3H.LG/Ckc. Does not cross react with other haplotypes (e.g., b, f, k, p, q, r, s).. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for H-2Dd Antibody (34-2-12) [DyLight 405LS]

• H2Dd
• MHC class I

Background

The 34-2-12 antibody recognizes alpha3 domain of H-2Dd MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dd haplotype. H-2Dd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-2-12 antibody cross-reacts with cells from the C3H.LG/Ckc strain. Reactivity with other haplotypes (e.g., b,f,k,p,q,r,s) has not been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.