GTPase Activity Kit (Colorimetric)



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GTPase Activity Kit (Colorimetric) Summary

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.

The kits contain our PiColorlock, a non-radioactive, superior phosphate detection reagent.

Key Features of PiColorLock:
  • Colorimetric Assay-competitor assays are radioactive.
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of phosphate-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.
Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.
Kit Type
Activity Kit (Colorimetric)


Read Publications using
602-0120 in the following applications:

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. 1 x 0.25 ml of Accelerator (1 x 0.5 ml)
  2. 1 x 1.5 ml of 0.1M MgCl2 (2 x 1.5 ml)
  3. 1 x 10 ml of PiColorLock (1 x 25 ml)
  4. 1 x 5 ml of 0.1mM Pi standard (1 x 10 ml)
  5. 1 x 5 ml of 0.5M Tris pH 7.5 (1 x 10 ml)
  6. 1 x 5 ml of Stabiliser (1 x 10 ml)
  7. 2 x 96-well plates (5 plates)
  8. 4 vials of lyophilized ATP (10 vials)


This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact with questions.


This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening. Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate. At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance. Uniquely, Innova Biosciences GTPase assay kits contain highly purified GTP, which have had any free inorganic phosphate removed ® this means that assays have the lowest possible background signal. PiColorLock reagent may also be purchased separately for developing simple assays for any phosphate-generating enzyme. It is offered in both high (Gold) and standard sensitivity (ALS) formulations to suit almost any assay situation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for GTPase Kit (602-0120)(40)

We have publications tested in 4 confirmed species: Human, Mouse, E. coli, Fish.

We have publications tested in 3 applications: EnzAct, Func, In vitro.

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In vitro
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E. coli
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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using 602-0120 Applications Species
Sarkar C, Sadhukhan T, Bagh MB et al. Cln1-mutations suppress Rab7-RILP interaction and impair autophagy contributing to neuropathology in a mouse model of infantile neuronal ceroid lipofuscinosis J. Inherit. Metab. Dis. 2020-04-12 [PMID: 32279353]
Cherubini M, Puigdellivol M, Alberch J, Gines S et al. Cdk5-mediated mitochondrial fission: A key player in dopaminergic toxicity in Huntington's disease Biochim. Biophys. Acta. 2015-07-02 [PMID: 26143143] (Mouse) Mouse
Yan J, Liu XH, Han MZ et al. Blockage of GSK3 beta-mediated Drp1 phosphorylation provides neuroprotection in neuronal and mouse models of Alzheimer’s disease. Neurobiol Aging 2014-01-01 [PMID: 25192600]
Li W, Feng J, Gao C et al. Nitration of Drp1 provokes mitophagy activation mediating neuronal injury in experimental autoimmune encephalomyelitis Free Radical Biology and Medicine 2019-08-01 [PMID: 31377418] (EnzAct) EnzAct
Ferreira JCB, Campos JC, Qvit N et al. A selective inhibitor of mitofusin 1-beta IIPKC association improves heart failure outcome in rats. Nat Commun. 2019-04-09 [PMID: 30659190] (In vitro) In vitro
Hoque A, Sivakumaran P, Bond ST et al. Mitochondrial fission protein Drp1 inhibition promotes cardiac mesodermal differentiation of human pluripotent stem cells. Cell Death Discov. 2018-12-01 [PMID: 29531836] (Human) Human
Lin SJ, Chiang MC, Shih HY et al. Regulator of G protein signaling 2 (Rgs2) regulates neural crest development through Ppard-Sox10 cascade. Biochim. Biophys. Acta. 2016-12-13 [PMID: 27979767] (Func, Fish) Func Fish
Franco A, Kitsis RN, Fleischer JA et al. Correcting mitochondrial fusion by manipulating mitofusin conformations. Nature. 2016-12-01 [PMID: 27775718]
Ho DH, Jang J, Joe EH et al. G2385R and I2020T Mutations Increase LRRK2 GTPase Activity. Biomed Res Int. 2016-01-01 [PMID: 27314038]
Show All 40 Publications.

Reviews for GTPase Kit (602-0120) (0)

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FAQs for GTPase Kit (602-0120). (Showing 1 - 7 of 7 FAQs).

  1. May we have the protocol for 602-0120? The GTPase assay kit protocol seems insufficient.
    • Here is the corresponding full protocol for your customer for this kit. I hope they find this information useful.
  2. I have phosphate in my enzyme. What can I do?
    • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
  3. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
    • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
  4. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
    • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
  5. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
    • Yes, only the phosphate will give a signal; pyrophosphate will not.
  6. This is regarding the GTPase assay kit catalog No: 602-0120. I want to know what is the equipment one needs to take the reading as kit contents are pipetted out in a 96-well plate. Does this require a fluorescence plate reader or will it be okay to take reading on the spectrophotometer if a cuvette is used.
    • It is possible to carry out the detection step using cuvettes, but sufficient volume for the cuvette must be used, and note that the PiColorLock solution may damage the cuvette.
  7. At which temperature does the assay have to be performed? 
    • The assay can be carried out at room temperature, as the kit contains proprietary buffers which reduce background hydrolysis (the assay is less temperature sensitive because of this). Please note that it is important for all reagents to be warmed up to room temperature before the assay is carried out.

Blogs on GTPase.

Understanding OPA1 and Mitochondrial Function
OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.

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