GSK3 alpha and GSK3 beta Pathway Assay Kit

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-71670
    • Availability
      Product Discontinued

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GSK3 alpha and GSK3 beta Pathway Assay Kit Summary

Description
This ELISA product range provides a quick, easy, more sensitive, and qualitatively better alternative to western blotting for kinase assay readouts, and it is designed to demonstrate the qualitative differences in the amounts of specific target proteins between samples.
Specificity
Kit contains (1) 96 well strip plate containing GSK3 alpha (pS21) and GSK beta (pS9), 3 pairs of strips each (12 strips total) with a kit assay control for the qualitative detection of phosphorylated GSK3 alpha & GSK3 beta in tissue lysates, cell lysates and recombinant protein.
Kit Type
Pathway Assay Kit

Applications/Dilutions

Dilutions
  • Immunoassay

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 2 x 8-microwell strips
  2. 20x Wash buffer Concentrate
  3. 5x Sample Dilution Buffer Concentrate
  4. Antibody-Coated Microwells
  5. Assay Control
  6. Biotin-Conjugated Detection Antibody
  7. Microwell Strip Holder
  8. Plate Cover
  9. Stop Solution
  10. Streptavidin Horseradish Peroxidase
  11. Tetramethylbenzidine Substrate

Notes

You may also Mix-and-Match you own Pathway Assay Kit by purchasing catalog number NBP1-71668. Choose up to 6 different targets from this list: Active Beta Catenin (DP S33/S37/T41), AKT1 (pS473), AKT2 (pS474), CREB (pS133), ERK1/2 (pT202/Y204; pT185/Y187), GSK3 alpha (pS21), GSK3 beta (pS9), p38 (pT180/Y182).

View All Available Pathway Assay Kits

Background

Multi-kinase arrays are an alternative analysis platform to traditional Western blots. Novus' Pathway Assay Kits allow for the simultaneous detection of activation states of multiple kinases in a signal pathway on a single multiwell platform. These kits are easier and faster than traditional Western blots (2.5 hour protocol), demonstrate a significantly greater dynamic range and sensitivity, operate with much less sample (5-20 micrograms total protein), and are more cost-effective with up to six data points per assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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