Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Protein

Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein [B-1205-NB] - Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, Biotinylated

• Reactivity: Pl
• Applications: WB, DB, ELISA, ICC/IF, IHC, Bioactivity, ELISA
• Conjugate: Biotin

Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Protein Summary

• Description: A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.
  

  Source: Griffonia (Bandeiraea) simplicifolia seeds
  
  GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.
  
  Biotinylated GSL I-B4 has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
  Specific for galactose.

• Details of Functionality: This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.
• Protein/Peptide Type: Protein
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Dot Blot
  • ELISA
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: For most applications, a freshly prepared working solution of 5 - 20 ug/mL in the buffer mentioned below is recommended..
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mM CaCl2
• Preservative: 0.08% Sodium Azide
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Protein (B-1205-NB)(1)

Publication using B-1205-NB

• Sen T, Cawthon CR, Ihde B, et al. Diet-driven microbiota dysbiosis is associated with vagal remodeling and obesity. Physiol Behav. [PMID: 28249783] (IF, Rat)