Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

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Product Details

Summary
Reactivity PlSpecies Glossary
Applications WB, DB, ICC/IF, IHC, Bioactivity
Concentration
LYOPH

Order Details

Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein Summary

Description
A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.

Source: Griffonia (Bandeiraea) simplicifolia seeds

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.

Details of Functionality
This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.
Protein/Peptide Type
Native Protein
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • Bioactivity
  • Dot Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
Lyophilized from water
Preservative
No Preservative
Concentration
LYOPH
Purity
Affinity purified
Reconstitution Instructions
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+ is recommended.

Notes

For reconstituted liquid, storage at 4 C with 0.08% sodium azide can be used. For preserving solutions stored at 4C, 0.08% sodium azide can be used. Specific for galactose. Inhibitors: 500 mM galactose or 100 mM raffinose .

Background

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from non-primates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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