Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

• Reactivity: Pl
• Applications: ICC/IF, Bioactivity
• Conjugate: FITC

Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein Summary

• Description: A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.
  

  Source: Griffonia (Bandeiraea) simplicifolia seeds
  
  GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.
  
  Fluorescein labeled GSL I-B4 has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorescein and is preserved with sodium azide. Excitation maximum = 495 nm; emission maximum = 515 nm. Color = green.

• Details of Functionality: This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.
• Protein/Peptide Type: Native Protein
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: For most applications, the recommended concentration range is 5 - 20 ug/mL. For immunofluorescence applications, it is recommended that sections be mounted in VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) or VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI (H-1500-NB)..
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mM Ca2+
• Preservative: 0.08% Sodium Azide
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.