Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

• Reactivity: Pl
• Applications: ICC/IF, Bioactivity
• Conjugate: DyLight 594

Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein Summary

• Description: A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.
  

  Source: Griffonia (Bandeiraea) simplicifolia seeds
  
  GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.
  
  DyLight® 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight® 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.
  
  Excitation maximum is at 592 nm and emission maximum is at 617 nm (red).
  Specific for galactose. Inhibitors: 500 mM galactose or 100 mM raffinose

• Details of Functionality: This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.
• Protein/Peptide Type: Native Protein
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: For most applications, the recommended concentration range is 5 - 20 ug/mL. For immunofluorescence applications, it is recommended that sections be mounted in VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) or VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI (H-1500-NB)..
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mM Ca2+
• Preservative: 0.08% Sodium Azide
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

DyLight(R) 594 GSL I-B4 isolectin is produced by using special conjugation procedures to incorporate DyLight(R) 594 into our affinity-purified lectin. This conjugate has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This reagent is supplied essentially free of unconjugated fluorochromes and inactive lectin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.