Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

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Product Details

Summary
Reactivity PlSpecies Glossary
Applications ICC/IF, Bioactivity
Conjugate
DyLight 594

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Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein Summary

Description
A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.

Source: Griffonia (Bandeiraea) simplicifolia seeds

Details of Functionality
This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.
Protein/Peptide Type
Native Protein
Purity
Affinity purified

Applications/Dilutions

Application Notes
For most applications, the recommended concentration range is 5-20 ug/ml. For immunofluorescence applications, it is recommended that sections be mounted in VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) or VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium with DAPI (H-1500-NB).
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM Ca2+
Preservative
0.08% Sodium Azide
Purity
Affinity purified

Notes

Specific for galactose. Inhibitors: 500 mM galactose or 100 mM raffinose

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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