Granzyme B Activity Assay Kit (Fluorometric) Summary
Fluorometric assay to measure Granzyme B activity and characterize Granzyme B inhibitors.
Detection method: Fluorescence microscopy, fluorescence plate reader (Ex/Em = 380/500 nm) and Flow cytometryApplications: Measurement of Granzyme B activity in various biological samples or purified Granzyme B, screening and characterizing Granzyme B inhibitors.
This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab157403)
Assay Kit (Fluorometric)
Packaging, Storage & Formulations
- AFC Standard (1mM)
- Granzyme B Assay Buffer
- Granzyme B Substrate
- Positive Control (human recombinant Granzyme B enzyme)
Granzyme B (GZMB, EC number 220.127.116.11) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In The Granzyme B Activity Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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