Granzyme B Activity Assay Kit (Fluorometric)

• Reactivity: Hu, Mu, Rt, Ma
• Suitable Sample Type: Various biological samples

Granzyme B Activity Assay Kit (Fluorometric) Summary

• Description: Fluorometric assay to measure Granzyme B activity and characterize Granzyme B inhibitors.
  
  Detection method: Fluorescence microscopy, fluorescence plate reader (Ex/Em = 380/500 nm) and Flow cytometryApplications: Measurement of Granzyme B activity in various biological samples or purified Granzyme B, screening and characterizing Granzyme B inhibitors.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab157403)
• Kit Type: Assay Kit (Fluorometric)

Applications/Dilutions

Publications

Read Publications using NBP2-54853.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In The Granzyme B Activity Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Granzyme B Activity Kit (NBP2-54853)(2)

Publications using NBP2-54853

• Leland P, Kumar D, Nimmagadda S et al. Characterization of chimeric antigen receptor modified T cells expressing scFv-IL-13R?2 after radiolabeling with 89Zirconium oxine for PET imaging Journal of translational medicine 2023-06-07 [PMID: 37286997]
• Pourzia AL, Olson ML, Bailey SR et al. Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells Cell death & disease 2023-04-13 [PMID: 37055388]