GPR15L Antibody (1020903) [Unconjugated]

Recombinant Human C10orf99 protein was serially diluted 2-fold and captured by Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB10416) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB104161) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 1020903
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR15L Antibody (1020903) [Unconjugated] Summary

• Immunogen: E. coli-derived human C10orf99
• Specificity: Detects human C10orf99 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR15L Antibody (1020903) [Unconjugated]

• AP57
• AP-57
• C10orf99 chromosome 10 open reading frame 99
• C10orf99
• GPR15L
• UNQ1833

Background

Human Chromosome 10 open reading frame 99 is a 9.2 KDa protein encoded by the C10orf99 gene.  C10orf99 has been described as a potential cytokine and a ligand Sushi Domain Containing 2 (SUSD2), inducing SUSD2 internalization upon binding. It has also been proposed to be a chemotactic factor that mediates lymphocytes recruitment to epithelia through binding and activation of the G-protein coupled receptor GPR15.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.