Glyoxalase 1 Activity Activity Assay Kit (Colorimetric)

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Product Details

Summary
Reactivity MaSpecies Glossary
Suitable Sample Type
Tissue and cell culture lysate

Order Details

Glyoxalase 1 Activity Activity Assay Kit (Colorimetric) Summary

Description
The kit utilizes the ability of an active GloI to catalyze the formation of SLG by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm. Detection of Glyoxalase I activity as low as 0.05 mU in biological samples.
Kit Type
Activity Assay Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.

Kit Components

Components
  1. GloI Assay Buffer
  2. GloI Substrate A
  3. GloI Substrate B (Lyophilized)
  4. Glyoxalase I (Lyophilized)
  5. U.V. transparent plate (96-well)

Background

Glyoxalase I (GloI, lactoylglutathione lyase, EC 4.4.1.5) is a cytosolic enzyme that participates in the Glyoxalase system. The glyoxalase system is a metabolic pathway that detoxifies a-ketoaldehydes such as methylglyoxal to D-lactic acid, via the intermediate S-D-lactoylglutathione (SLG). Specifically, GloI catalyzes the formation of SLG from methylglyoxal and reduced Glutathione. It is overexpressed in several human cancers such as colon, breast, prostate and melanoma. GloI also enhances resistance to antitumor agent-induced apoptosis in human leukemia cells. In addition, recent findings have linked GloI to numerous anxiety-like behaviors including psychiatric diseases (depression, schizophrenia and autism) and pain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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