Glycine R Antibody [Unconjugated] - Pan Specific

Western blot of 10 μg of rat spinal cord. As shown in the autoradiograph, the Anti-Glycine Receptor antibody is specific for the ~48 kDa subunits recognizing both the alpha 1 and alpha 2 subunits of the Glycine Receptor.

Product Discontinued

Glycine R Antibody [Unconjugated] - Pan Specific Summary

• Immunogen: Peptide from the N-terminus region of the alpha 1 subunit of the rat Glycine Receptor
• Specificity: Specific for the ~48 kDa alpha 1 and alpha 2 subunits of the Glycine Receptor in Western blots of rat spinal cord, brain stem and cell extracts. Immunolabeling is blocked by preadsorption of antibody with the peptide immunogen. This antibody does not recognize other Glycine Receptor subunits.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 dilution
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: The lyophilized product is stable at ≤- 20° C for at least 1 year. After reconstitution the antibody should be aliquoted and stored at ≤ -20° C.
• Buffer: Lyophilized in 5 mM ammonium bicarbonate
• Preservative: No Preservative
• Reconstitution Instructions: This antibody should be reconstituted in 100 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glycine R Antibody [Unconjugated] - Pan Specific

• Glycine R

Background

The rat glycine receptor (GlyR) chloride channel is a member of the nicotinic acetylcho-line receptor family of ligand-gated ion channels. Its glycine-mediated activation results in an influx of chlorine ions that hyperpolarize and desensitize neuronal postsynaptic membranes. The receptor’s multimeric makeup is unclear. It may be either a three or five subunit homo- or heteromultimer. To date, there are five rat GlyR subunit types. Four are 48-53 kDa alpha -type subunits, and one is a 58 kDa beta -type subunit. Alternate splicing exists for alpha 1 and alpha 2 subunit types. Alpha-type subunits may form homotrimers; however, their function may not be neurotransmission but rather non-synaptic cell-cell communication. Alpha1-beta multimers may be the principal synaptic GlyR in adults.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.