Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free

Western Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Recombinant GST (0.2ug) [+]. Lane 3: HEK293T WCL / rec. GST(10ug/0.2ug) [+]. Lane 4: HEK293T Whole Cell Lysate (10ug) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (10ug) [-]. Primary Antibody: Anti-GST (VhH) at 5.0ug/mL overnight at 2-8 degrees C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:3,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 60 sec. Predicted MW: ~25.5kDa. Observed MW: ~25.5kDa. Unspecific Bands: ~70kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.

Dot Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Row A: Recombinant GST protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.5hr at RT. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:500 for 30 mins at RT. Block: BlockOut Buffer.

• Reactivity: Ec
• Applications: WB, DB, IHC
• Clone: GST13
• Clonality: Monoclonal
• Host: Llama
• Conjugate: Unconjugated
• Format: BSA Free

Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free Summary

• Description: This recombinant antibody clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture (E. coli) .
  
  Store at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Glutathione S-Transferase/GST
• Specificity: Expect reactivity against GST-labeled probes and proteins.
• Clonality: Monoclonal
• Host: Llama
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:200
• Application Notes: This product is a his-tagged monoclonal recombinant antibody designed to detect Glutathione-S-Transferase and its conjugates. This antibody has been tested by western blot and dot blot and is intended for use in immunological assays including western blotting, immunofluorescence and fluorescence activated cell sorting (FACS). Fluorochrome conjugated anti-GST can be used for detection of sensitive non-radioactive in situ hybridization probes. The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of GST-conjugated molecules by immunoblotting or immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 1X PBS, pH 7.42, 20% (v/v) Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Glutathione S-Transferase/GST Antibody (GST13) - VHH - BSA Free

• Glutathione STransferase
• Glutathione S-Transferase

Background

For the highly sensitive and specific detection of GST-tagged recombinant proteins in Western blots and ELISAs. In an indirect chemiluminescence system this product is quality tested todetect 10 ng of purified GST-tagged ubiquitin (N-terminal tag).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.