Glutathione Assay Kit (Colorimetric)

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Glutathione Assay Kit (Colorimetric) [KA0797] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA0797
    • Availability
      Product Discontinued

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Glutathione Assay Kit (Colorimetric) Summary

Description
Quality control test: Standard curve Glutathione Assay Kit (Colorimetric) is used for analyzing total glutathione and the reduced form glutathione alone.
Specificity
This kit can quantify glutathione from 1-100 ng/well in a 200 uL reaction. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will generate stronger signals. This kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutathione.
Assay Type
Colorimetric
Sample Volume
20 uL
Kit Type
Assay Kit (Colorimetric)
Gene
GSH

Applications/Dilutions

Dilutions
  • Functional
  • Quantification
Publications
Read Publication using KA0797.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. Glutathione Reaction Buffer
  2. Glutathione Reductase
  3. Glutathione Substrate (DTNB)
  4. GSH Standard (lyophilized, MW 307)
  5. NADPH Generating Mix (lyophilized)
  6. Sulfosalicylic Acid (SSA, 1 gram)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glutathione Assay Kit (Colorimetric)

  • Glutathione

Background

DTNB and glutathione (GSH) react to generate 2-nitro-5-thiobenzoic acid which has yellow color. Therefore, GSH concentration can be determined by measuring absorbance at 412 nm. The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. Therefore, the recycling system dramatically improves the sensitivity of total glutathione detection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Glutathione Kit (KA0797)(1)

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Additional Glutathione Products

Array KA0797

Blogs on Glutathione.

Glutathione and xCT: Chemoresistance in Tumor Cells
Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.

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Bioinformatics

Gene Symbol GSH