Recombinant S. aureus Glutamyl endopeptidase Protein

SDS-Page: Recombinant S. aureus Glutamyl endopeptidase Protein [NBP2-76180] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel. Predicted MW of 28.9 kDa, but runs ~35 kDa.

Product Discontinued

Recombinant S. aureus Glutamyl endopeptidase Protein Summary

• Description: For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
  
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: Glutamyl endopeptidase is a monomer, 28.9 kDa (267 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
• Theoretical MW: 28.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant S. aureus Glutamyl endopeptidase Protein

• Endoproteinase Glu-C
• Staphylococcal serine proteinase
• V8 protease

Background

Glutamyl endopeptidase (Glu-C) is an enzyme from Staphylococcus aureus strain V8 that hydrolizes peptide bonds formed on the carboxyl terminal side of aspartate and glutamate amino acid residues. Glu-C is pathogenic to human tissue and functions during adherence and colonization of host cells. Glu-C protects against host defense mechanisms by fragmenting human immunoglobulins IgG, IgM, and IgA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.