Glucose Uptake Assay Kit (Fluorometric)

Glucose Uptake Assay Kit (Fluorometric) [NBP3-25776] - Standard Curve Reference

Glucose Uptake Assay Kit (Fluorometric) [NBP3-25776] - Assay Example

• Reactivity: All-NA
• Applications: Func
• Suitable Sample Type: Cells
• Standard Curve Range: 0.02-0.3 nmol/ul
• Sensitivity: 0.02 nmol/ul

Glucose Uptake Assay Kit (Fluorometric) Summary

• Description: Detection principle: 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.
  
  Detection method: Fluorescence method
  
  Other instruments required: Micropipettor, Incubator, Water bath
  
  Other reagents required: KRPH solution (20 mM Hepes, 5 mM KH2PO4, 1 mM MgSO4, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 2% BSA, pH 7.4)
• Standard Curve Range: 0.02-0.3 nmol/ul
• Sensitivity: 0.02 nmol/ul
• Assay Type: Quantitative
• Kit Type: Assay Kit (Fluorometric)

Applications/Dilutions

• Dilutions:
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.