Glu-Glu Epitope Tag Antibody

Product Discontinued

Glu-Glu Epitope Tag Antibody Summary

• Immunogen: Chickens were immunized with glu-glu cleavage site (EYMPME) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Chicken anti-human glu-glu affinity purified antibodies were coupled to peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Chicken
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1-4 ug/mg lysate
  • Western Blot 1:1000 - 1:5000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glu-Glu Epitope Tag Antibody

• EYMPME epitope tag
• EYMPME tag
• Glu-Glu epitope tag

Background

The Glu-Glu epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized Glu-Glu epitope represents amino acid residues 314-319, EYMPME, of middle T antigen of mouse polyomavirus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glu-Glu Epitope Tag Antibody (NB600-352)(1)

Publication using NB600-352

• Sakakibara N, Mitra R, McBride AA. The papillomavirus E1 helicase activates a cellular DNA damage response in viral replication foci. J Virol 2011-09-01 [PMID: 21734054]