Human Gliadin peptide ELISA Kit (Colorimetric) Summary
| Description |
Gliadin IgA Ab ELISA Kit is an indirect solid phase enzyme immunoassay for the quantitative measurement of specific IgA antibodies against Gliadin in serum and plasma. |
| Assay Type |
Indirect ELISA |
| Inter-Assay |
CV% < 10.10% |
| Intra-Assay |
CV% < 9.60% |
| Spike Recovery |
70-119% |
| Sample Volume |
100 uL (diluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Calibrator A (Negative Control): Protein solution diluted with PBS, contains no IgAantibodies against Gliadin. Addition of 0.01% methylisothiazolone and 0.01%bromonitrodioxane. Ready-to-use
- Calibrator B (Cut-Off Standard): Human serum diluted with PBS, contains a lowconcentration of IgA antibodies against Gliadin. Addition of 0.01% methylisothiazoloneand 0.01% bromonitrodioxane. Ready-to-use
- Calibrator C (Weak Positive Control): Human serum diluted with PBS, contains amedium concentration of IgA antibodies against Gliadin. Addition of 0.01%methylisothiazolone and 0.01% bromonitrodioxane. Ready-to-use
- Calibrator D (Positive Control): Human serum diluted with PBS, contains a highconcentration of IgA antibodies against Gliadin. Addition of 0.01% methylisothiazoloneand 0.01% bromonitrodioxane. Ready-to-use
- Enzyme Conjugate: Anti-human-IgA-HRP (rabbit), in protein-containing buffer solution.Ready-to-use
- Microtiter strips: Coated with a Gliadin antigen (purified gluten antigen from wheat).Ready-to-use
- Plastic bag: Resealable, for the dry storage of non-used strips.
- Sample Diluent: PBS/BSA buffer. Addition of 0.095% sodium azide. Ready-to-use
- Stop Solution: 1 N acidic solution. Ready-to-use
- Substrate Solution: TMB (tetramethylbenzidine). Ready-to-use.
- Washing Buffer (10x): PBS + Tween 20, 10x concentrate. Final concentration: dilute 1+9with distilled water. If during the cold storage crystals precipitate, the concentrate shouldbe warmed up at 37°C for 15 minutes.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Background
Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Gliadin peptide ELISA Kit (KA1286). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
![ELISA: Gliadin peptide ELISA Kit [HRP] [KA1286] - Standard curve](http://images.novusbio.com/fullsize/Gliadin-peptide-ELISA-Kit-[HRP]-ELISA-KA1286-img0001.jpg "ELISA: Gliadin peptide ELISA Kit [HRP] [KA1286] - Standard curve")