Gliadin peptide Antibody (4F3) - BSA Free

Western Blot: Gliadin peptide Antibody (4F3) [NBP1-05104] - Gliadin peptide (detected with NBP1-05104) in wheat (lane 1) and barley (lane 2).

Product Discontinued

Gliadin peptide Antibody (4F3) - BSA Free Summary

• Immunogen: Gliadin-related peptide Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) coupled to immunogenic carrier protein.
• Epitope: The epitope is located in the Lys57-Glu65-[alpha-gliadin (58-73)] (KLQPFPQPELPYPQPQ) peptide.
• Specificity: NBP1-05104 reacts equally strong with both the deamidated peptide (KLQPFPQPELPYPQPQ) and the non-deamidated peptide (KLQPFPQPQLPYPQPQ) when coated directly in an immunoplate.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Western Blot 1:100-1:2000
• Application Notes: Western blot data from customer review. Sandwhich ELISA is not recommended for this product, please use direct ELISA. Store at 4-8C without exposure to light No precautions necessary during handling.

Reactivity Notes

Cross-react with Gliadin Peptide in Wheat, Barley and Rye (data not shown for Rye). Does not react with Oats (data not shown).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Background

Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Gliadin peptide Antibody (NBP1-05104) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Susan de Thanch Roennenkamp WB Mouse 12/16/2013

Summary

• Application: Western Blot
• Species: Mouse
• Lot: F0915