Recombinant Drosophila GAGA-POZ domain Protein

SDS-Page: GAGA-POZ domain Protein [NBC1-18417] - GAGA-POZ domain, 14 kDa (130 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Dr
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Drosophila GAGA-POZ domain Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-130 of Drosophila melanogaster GAGA-POZ domain
  
  

  Source: E.coli

  Amino Acid Sequence: MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: Trl
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES buffer (pH7.4), 25 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Background

The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo. GAGA-POZ domain was overexpressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: Trl