Gag polyprotein Antibody

Western Blot: Gag polyprotein Antibody [NBP1-78117] - FIV-infected feline CrFK cells (lane 2, arrowhead). Lane 1 is an uninfected control. Virions were enriched by ultracentrifugation, lysed, resolved by electrophoresis, and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:10,000 dilution. Personal Communication, B. Luttge, CCR-NCI, Frederick, MD.

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Gag polyprotein Antibody Summary

• Immunogen: This affinity purified Gag polyprotein Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Gag polyprotein. (Uniprot: P16087)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Immunohistochemistry
  • Western Blot 1:1000-1:10000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, western blotting and immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding - FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive against Gag polyprotein from Feline Immunodeficiency Virus (FIV).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.22 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Gag polyprotein Antibody

• Pr55Gag

Background

Feline immunodeficiency virus (FIV) belongs to the lentivirus family. This family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes. The capsid assembles from the viral Gag polyprotein. Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid. Matrix Protein, located at the N-terminus of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly. The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes. Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides. In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle. Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.