g-linked GTP agarose resin

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Summary

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g-linked GTP agarose resin Summary

Description
Affinity resins have been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules. GTP-agarose resin comprises GTP attached to agarose beads via its gamma-phosphate. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. The ligand is coupled through the gamma-phosphate group which means that the resin is resistant to phosphatases found in many crude tissue extracts.

The Control Resin is just the agarose with no GTP bound.
Specificity
Affinity resins have been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules. GTP-agarose resin comprises GTP attached to agarose beads via its gamma-phosphate. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. The ligand is coupled through the gamma-phosphate group which means that the resin is resistant to phosphatases found in many crude tissue extracts.

The Control Resin is just the agarose with no GTP bound.

Applications/Dilutions

Application Notes
High (>6 umol/ml) - 5ml
Publications
Read Publications using 505-0002.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Notes

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Background

Our GTP agarose resin contains GTP linked via the terminal phosphate group. The resin has a long (14-atom) hydrophilic spacer to facilitate interactions with biomolecules. Linkage of GTP via the terminal phosphate means that the immobilised ligand cannot be removed by phosphatases in crude extracts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for g-linked GTP agarose resin (505-0002)(11)


Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 505-0002 Applications Species
Li Z, Ding B, Zhou X, Wang GL. The Rice Dynamin-Related Protein OsDRP1E Negatively Regulates Programmed Cell Death by Controlling the Release of Cytochrome c from Mitochondria. PLoS Pathog. 2017 Jan 12 [PMID: 28081268]
Jimenez JI, Xulvi-Brunet R, Campbell GW et al. Comprehensive experimental fitness landscape and evolutionary network for small RNA. PNAS 2013 [PMID: 23980164]
Salter NW, Ande SR, Nguyen HK et al. Functional Characterization of Naturally Occurring Transglutaminase 2 Mutants Implicated in Early-Onset Type 2 Diabetes. J Mol Endocrinol 2012 [PMID: 22394545]
Ito G, Iwatsubo T. Re-examination of the dimerization state of leucine-rich repeat kinase 2:predominance of the monomeric form. Biochem J. 2011 [PMID: 22047502]
Pendin D, Tosetto J, Moss TJ et al. GTP-dependent packing of a three-helix bundle is required for atlastin-mediated fusion. PNAS 2011 [PMID: 21930898]
Marino SF. High-level production and characterization of a G-protein coupled receptor signaling complex. FEBS J. 2009 [PMID: 19645726]
Green JC, Kahramanoglou C, Rahman A et al. Recruitment of the Earliest Component of the Bacterial Flagellum to the Old Cell Division Pole by a Membrane-Associated Signal Recognition Particle Family GTP-Binding Protein. J Mol Biol 2009 [PMID: 19497327]
Garcia-Regalado A, Guzman-Hernandez ML, Ramirez-Rangel I et al. G Protein-Coupled Receptor-Promoted Trafficking of G beta-1-gamma-2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by G?1?2-Rab11a Interaction. Mol Biol Cell 2008 [PMID: 18701709]
Yang Y, Rao R, Shen J, Tang Y, Fiskus W, Nechtman J, Atadja P, Bhalla K. Role of acetylation and extra-cellular location of heat shock protein 90a in tumor cell invasion. Cancer Research, 68 (12) pp 4833-4842. 2008 [PMID: 18559531]
Baloh RH, Schmidt RE, Pestronk A, Milbrandt J. Altered axonal mitochondrial transport in the pathogenesis of Charcot-Marie-Tooth disease from mitofusin 2 mutations. J Neurosci27(2):422-30. 2007 Jan 10. [PMID: 17215403]
Show All 11 Publications.

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