g-linked GTP agarose resin

Product Discontinued

g-linked GTP agarose resin Summary

• Description: Affinity resins have been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules. GTP-agarose resin comprises GTP attached to agarose beads via its gamma-phosphate. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. The ligand is coupled through the gamma-phosphate group which means that the resin is resistant to phosphatases found in many crude tissue extracts.
  
  The Control Resin is just the agarose with no GTP bound.

Applications/Dilutions

Publications

Read Publications using 505-0002.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.12% Tris (pH 8.0), 1.74% Sodium chloride, 0.03% EDTA, 5% Guanosine 5'-triphosphate lithium salt

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals. It was previously called High Capacity GTP Agarose - 2ml (505-0002); it is the same as the 2 mL size of this product.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Our GTP agarose resin contains GTP linked via the terminal phosphate group. The resin has a long (14-atom) hydrophilic spacer to facilitate interactions with biomolecules. Linkage of GTP via the terminal phosphate means that the immobilised ligand cannot be removed by phosphatases in crude extracts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for g-linked GTP agarose resin (505-0002)(11)

Publications using 505-0002

• Li Z, Ding B, Zhou X, Wang GL. The Rice Dynamin-Related Protein OsDRP1E Negatively Regulates Programmed Cell Death by Controlling the Release of Cytochrome c from Mitochondria. PLoS Pathog. 2017-01-12 [PMID: 28081268]
• Jimenez JI, Xulvi-Brunet R, Campbell GW et al. Comprehensive experimental fitness landscape and evolutionary network for small RNA. PNAS 2013-01-01 [PMID: 23980164]
• Salter NW, Ande SR, Nguyen HK et al. Functional Characterization of Naturally Occurring Transglutaminase 2 Mutants Implicated in Early-Onset Type 2 Diabetes. J Mol Endocrinol 2012-01-01 [PMID: 22394545]
• Ito G, Iwatsubo T. Re-examination of the dimerization state of leucine-rich repeat kinase 2:predominance of the monomeric form. Biochem J. 2011-01-01 [PMID: 22047502]
• Pendin D, Tosetto J, Moss TJ et al. GTP-dependent packing of a three-helix bundle is required for atlastin-mediated fusion. PNAS 2011-01-01 [PMID: 21930898]
• Marino SF. High-level production and characterization of a G-protein coupled receptor signaling complex. FEBS J. 2009-01-01 [PMID: 19645726]
• Green JC, Kahramanoglou C, Rahman A et al. Recruitment of the Earliest Component of the Bacterial Flagellum to the Old Cell Division Pole by a Membrane-Associated Signal Recognition Particle Family GTP-Binding Protein. J Mol Biol 2009-01-01 [PMID: 19497327]
• Garcia-Regalado A, Guzman-Hernandez ML, Ramirez-Rangel I et al. G Protein-Coupled Receptor-Promoted Trafficking of G beta-1-gamma-2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by G?1?2-Rab11a Interaction. Mol Biol Cell. 2008-01-01 [PMID: 18701709]
• Yang Y, Rao R, Shen J et al. Role of Acetylation and Extracellular Location of Heat Shock Protein 90alpha in Tumor Cell Invasion. Cancer Res 2008-01-01 [PMID: 18559531]
• Baloh RH, Schmidt RE, Pestronk A, Milbrandt J. Altered axonal mitochondrial transport in the pathogenesis of Charcot-Marie-Tooth disease from mitofusin 2 mutations. J Neurosci27(2):422-30. 2007-01-10 [PMID: 17215403]
• Rao MR, Kumari G, Balasundaram D et al. A Novel Lysine-rich Domain and GTP Binding Motifs Regulate the Nucleolar Retention of Human Guanine Nucleotide Binding Protein, GNL3L. J Mol Biol 2006-01-01 [PMID: 17034816]