g-linked ATP agarose resin

Product Discontinued

g-linked ATP agarose resin Summary

• Description: Affinity resins have been widely used for the purification of enzymes that bind nucleotides and related molecules. Resins in which ATP is linked via the gamma-phosphate have been valuable in identifying proteins in the purine-binding proteome, which includes kinases, heat shock proteins and other ATP-binding proteins.
  
  ATP Agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. Two forms of the resin are available with low and high ligand substitution. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules.
  
  The Control Resin is just the agarose with no ATP bound.
• Specificity: Affinity resins have been widely used for the purification of enzymes that bind nucleotides and related molecules. Resins in which ATP is linked via the gamma-phosphate have been valuable in identifying proteins in the purine-binding proteome, which includes kinases, heat shock proteins and other ATP-binding proteins.
  
  ATP Agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. Two forms of the resin are available with low and high ligand substitution. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules.
  
  The Control Resin is just the agarose with no ATP bound.

Applications/Dilutions

• Application Notes: High (8-12 umol/ml) - 5ml

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Background

ATP linked to a support via its g-phosphate has been used for affinity purification of kinases, heat-shock proteins and other ATP-binding proteins. KinaseBind is a g-phosphate-linked ATP resin with a long hydrophillic spacer that facilitates molecular interactions with biomolecules. Linkage of ATP via the terminal phosphate means that the immobilised ligand cannot be removed by phosphatases in crude extracts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for g-linked ATP agarose resin (510-0005)(4)

Publications using 510-0005

• Rao R, Fiskus W, Yang Y et al. HDAC6 inhibition enhances 17-AAG--mediated abrogation of hsp90 chaperone function in human leukemia cells. Blood. 2008-09-01 [PMID: 18591380]
• Duncan JA, Bergstralh DT, Wang Y, Willingham SB, Ye Z, Zimmermann AG, Ting JP. ryopyrin/NALP3 binds ATP/dATP, is an ATPase, and requires ATP binding to mediate inflammatory signaling. Proc Natl Acad Sci U S A. 104(19):8041-6. 2007-05-08 [PMID: 17483456]
• Harthill JE, Meek SE, Morrice N, Peggie MW, Borch J, Wong BH, Mackintosh C. Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose. The Plant Journal 47 (2), 211-223. 2006-07-01 [PMID: 16771775]
• Jaleel M, Saha S, Shenoy AR, Visweswariah SS. The kinase homology domain of receptor guanylyl cyclase C: ATP binding and identification of an adenine nucleotide sensitive site. Biochemistry;45(6):1888-98. 2006-02-14 [PMID: 16460035]