g-linked ATP agarose resin

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Summary

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g-linked ATP agarose resin Summary

Description
Affinity resins have been widely used for the purification of enzymes that bind nucleotides and related molecules. Resins in which ATP is linked via the gamma-phosphate have been valuable in identifying proteins in the purine-binding proteome, which includes kinases, heat shock proteins and other ATP-binding proteins.

ATP Agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. Two forms of the resin are available with low and high ligand substitution. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules.

The Control Resin is just the agarose with no ATP bound.
Specificity
Affinity resins have been widely used for the purification of enzymes that bind nucleotides and related molecules. Resins in which ATP is linked via the gamma-phosphate have been valuable in identifying proteins in the purine-binding proteome, which includes kinases, heat shock proteins and other ATP-binding proteins.

ATP Agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. Two forms of the resin are available with low and high ligand substitution. A long hydrophilic spacer (14-atom) is used to minimise unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules.

The Control Resin is just the agarose with no ATP bound.

Applications/Dilutions

Application Notes
High (8-12 umol/ml) - 2ml
Publications
Read Publications using 510-0002.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Notes

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Background

ATP linked to a support via its g-phosphate has been used for affinity purification of kinases, heat-shock proteins and other ATP-binding proteins. KinaseBind is a g-phosphate-linked ATP resin with a long hydrophillic spacer that facilitates molecular interactions with biomolecules. Linkage of ATP via the terminal phosphate means that the immobilised ligand cannot be removed by phosphatases in crude extracts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for g-linked ATP agarose resin (510-0002)(5)

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FAQs for g-linked ATP agarose resin (510-0002). (Showing 1 - 1 of 1 FAQs).

  1. What is the binding capacity of the ATP Agarose?
    • The binding of any ATP-binding protein to our resin will depend on a number of factors. These include amongst others: the formulation of the binding buffer, the incubation time of the sample with the resin (flow rate), the concentration of the ATP-binding protein, the affinity of the binding protein for ATP and the size of the binding protein (steric hindrance can be a factor with larger proteins). The only true way to establish the capacity of the column for any individual binding protein is by experimentation. If you require mg scale purification then our ATP agarose should be suitable, especially as the resin can be reused. The unique nature of our ATP resin is that it is not subject to dephosphorylation like many other similar resins available from other suppliers. This means that you can simply wash, re-equilibrate and reuse the resin multiple times. This will enable you to obtain mg quantities of your protein even if it takes more than one purification.

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