Mouse Free Thyroxine ELISA Kit (Colorimetric)

ELISA: Mouse Free Thyroxine ELISA Kit (Colorimetric) [NBP2-60001] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse Free Thyroxine ELISA Kit (Colorimetric) [NBP2-60001] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 4 pmol/L - 64 pmol/L
• Sensitivity: 1.08 pmol/L

Mouse Free Thyroxine ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FT4. No significant cross-reactivity or interference between mouse FT4 and analogues was observed.
• Standard Curve Range: 4 pmol/L - 64 pmol/L
• Sensitivity: 1.08 pmol/L
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Free Thyroxine ELISA Kit (NBP2-60001). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.