Rat Free Thyroxine ELISA Kit (Colorimetric)

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ELISA: Rat Free Thyroxine ELISA Kit (Colorimetric) [NBP2-60000] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
4 pmol/L-64 pmol/L
Sensitivity
1.08 pmol/L

Order Details

Rat Free Thyroxine ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of rat FT4. No significant cross-reactivity or interference between rat FT4 and analogues was observed.
Standard Curve Range
4 pmol/L-64 pmol/L
Sensitivity
1.08 pmol/L
Assay Type
Competitive ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. Conjugate
  4. HRP-avidin
  5. Instruction manual
  6. Standard
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT4 in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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