Francisella Tularensis LPS Antibody (T14)

Product Discontinued

Francisella Tularensis LPS Antibody (T14) Summary

• Immunogen: Hybridoma clone for MAb T14 has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with pooled water-soluble antigens from disintegrated microbial mass of Fr. tularensis vaccine strain.
• Specificity: Reacts with LPS of Fr. tularensis. There is no cross-reactivity with Y. pestis, Y. pseudotuberculosis, Y. enterocolitica, V. cholera, E. coli, S. typhimurium, Fr. novicida, Br. melitensis, Br. abortus, Br. suis, Br. ovis, and Br. neotomae.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000

Reactivity Notes

Bacteria.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Francisella Tularensis LPS Antibody (T14)

• Lipopolysaccharide protein
• Lipopolysaccharide

Background

Francisella tularensis is a small, nonmotile, aerobic, gram-negative coccobacillus. It has a thin lipopolysaccharide-containing envelope and is a hardy non-spore-forming organism that survives for weeks at low temperatures in water, moist soil, hay, straw, and decaying animal carcasses. Lipopolysaccaride (LPS) is a main species-specific antigen of Francisella tularensis. According to the data available LPS of tularemia microbe differs from LPS of other gram-negative bacteria. LPS of tularemia microbe hasn't yet revealed the properties of classical endotoxin, which may be connected with non-typical structure of lipid A - a toxic component of LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.