Description | Components Fixation/Permeabilization Concentrate: 30 ml. Store at 4 degrees C. Avoid agitation. Please use within expiration date indicated on the bottle. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Concentrate with 3 parts Diluent. Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves. Fixation/Permeabilization Diluent: 100 ml. Store at 4 degrees C. The diluent is intented to be used in combination with the Fixation/Permeabilization Concentrate. Permeabilization Buffer (10X): 100 ml. Store at 4 degrees C. Dilute to 1X with deionized/distilled water and store at 4 degrees C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution. |
Specificity | The Foxp3/Transcription Factor Staining Buffer Set have been formulated and optimized for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67 as well as cytokines and chemokines. |
Storage | Store at 4C in the dark. |
Buffer | PBS (pH 7.2) with 0.1% gelatin |