Bovine Folic acid ELISA Kit (Colorimetric)

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ELISA: Bovine Folic acid ELISA Kit (Colorimetric) [NBP2-59998] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity BvSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma, tissue homogenates
Standard Curve Range
0.39 ng/ml-100 ng/ml
Sensitivity
0.39 ng/ml

Order Details

Bovine Folic acid ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of bovine FA. No significant cross-reactivity or interference between bovine FA and analogues was observed.
Standard Curve Range
0.39 ng/ml-100 ng/ml
Sensitivity
0.39 ng/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Spike Recovery
92% (serum) and 98% (EDTA plasma)
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Standard
  2. Stop Solution
  3. Sample Diluent
  4. TMB Substrate
  5. Wash Buffer (25 x concentrate)
  6. Assay plate (12 x 8 coated Microwells)
  7. Biotin-conjugate (10 x concentrate)
  8. Biotin-conjugate Diluent
  9. HRP-avidin Diluent
  10. HRP-avidin (100 x concentrate)
  11. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Bovine Folic acid ELISA Kit (Colorimetric)

  • 5 Methyl THFA
  • 5 Methyltetrahydrofolate
  • Folate
  • Tetrahydrofolate
  • Tetrahydrofolic acid
  • THFA

Background

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for FA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated FA. A competitive inhibition reaction is launched between FA (Standards or samples) and biotin-conjugated FA with the pre-coated FA antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of FA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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