Rat Folic acid ELISA Kit (Colorimetric)

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ELISA: Rat Folic acid ELISA Kit (Colorimetric) [NBP2-59995] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Rat Folic acid ELISA Kit (Colorimetric) [NBP2-59995] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
19.5 pg/mL - 5000 pg/mL
Sensitivity
19.5 pg/mL

Order Details

Rat Folic acid ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of rat folic acid. No significant cross-reactivity or interference between rat folic acid and analogues was observed.
Standard Curve Range
19.5 pg/mL - 5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Antibody (100 x concentrate)
  3. Antibody Diluent
  4. Assay plate
  5. HRP-conjugate (100 x concentrate)
  6. HRP-conjugate Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Rat Folic acid ELISA Kit (Colorimetric)

  • Folate
  • Tetrahydrofolate
  • Tetrahydrofolic acid
  • THFA

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for folic acid and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated folic acid and folic acid in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of folic acid in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Folic acid ELISA Kit (NBP2-59995). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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