Rat Folic acid ELISA Kit (Colorimetric)

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ELISA: Rat Folic acid ELISA Kit (Colorimetric) [NBP2-59995] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
19.5 pg/ml-5000 pg/ml
Sensitivity
19.5 pg/ml

Order Details

Rat Folic acid ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of rat folic acid. No significant cross-reactivity or interference between rat folic acid and analogues was observed.
Standard Curve Range
19.5 pg/ml-5000 pg/ml
Sensitivity
19.5 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Instruction manual
  2. HRP-conjugate (100 x concentrate)
  3. HRP-conjugate Diluent
  4. Adhesive Strip (For 96 wells)
  5. Assay plate
  6. Antibody Diluent
  7. Antibody (100 x concentrate)
  8. Wash Buffer (25 x concentrate)
  9. TMB Substrate
  10. Sample Diluent
  11. Standard (Freeze dried)
  12. Stop Solution

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Rat Folic acid ELISA Kit (Colorimetric)

  • 5 Methyl THFA
  • 5 Methyltetrahydrofolate
  • Folate
  • Tetrahydrofolate
  • Tetrahydrofolic acid
  • THFA

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for folic acid and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated folic acid and folic acid in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of folic acid in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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