Human Folic acid ELISA Kit (Colorimetric)

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ELISA: Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
12.5 pg/mL - 200 pg/mL
Sensitivity
6.25 pg/mL

Order Details

Human Folic acid ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Standard Curve Range
12.5 pg/mL - 200 pg/mL
Sensitivity
6.25 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
Publications
Read Publication using NBP2-59966.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. HRP-conjugate (100 x concentrate)
  4. HRP-conjugate Diluent
  5. Instruction manual
  6. Sample Diluent
  7. Standard (10 x concentrate)
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (25 x concentrate)

Alternate Names for Human Folic acid ELISA Kit (Colorimetric)

  • Folate
  • Tetrahydrofolate
  • Tetrahydrofolic acid
  • THFA

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated FA. The competitive inhibition reaction is launched between with HRP-conjugated FA and FA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Folic acid ELISA Kit (NBP2-59966)(1)

We have publications tested in 1 application: ELISA.


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Publication using NBP2-59966 Applications Species
Alhuthali H, Almehmadi M, Ataya E et al. Neutrophilia and its correlation with increased inflammatory response in COVID-19 in diabetic and pre-diabetic patients European Journal of Inflammation 2023-12-01 (ELISA) ELISA

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FAQs for Folic acid ELISA Kit (NBP2-59966). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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