Human Folic acid ELISA Kit (Colorimetric)

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ELISA: Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
12.5 pg/mL - 200 pg/mL
Sensitivity
6.25 pg/mL

Order Details

Human Folic acid ELISA Kit (Colorimetric) Summary

Standard Curve Range
12.5 pg/mL - 200 pg/mL
Sensitivity
6.25 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Instruction manual
  2. HRP-conjugate Diluent
  3. HRP-conjugate (100 x concentrate)
  4. Adhesive Strip (For 96 wells)
  5. Assay plate
  6. TMB Substrate
  7. Wash Buffer (25 x concentrate)
  8. Stop Solution
  9. Sample Diluent
  10. Standard (10 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Human Folic acid ELISA Kit (Colorimetric)

  • 5 Methyl THFA
  • 5 Methyltetrahydrofolate
  • Folate
  • Tetrahydrofolate
  • Tetrahydrofolic acid
  • THFA

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated FA. The competitive inhibition reaction is launched between with HRP-conjugated FA and FA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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