ELISA: Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Folic acid ELISA Kit (Colorimetric) [NBP2-59966] - Standard Curve Reference
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Adhesive Strip (For 96 wells)
Assay plate
HRP-conjugate (100 x concentrate)
HRP-conjugate Diluent
Instruction manual
Sample Diluent
Standard (10 x concentrate)
Stop Solution
TMB Substrate
Wash Buffer (25 x concentrate)
Alternate Names for Human Folic acid ELISA Kit (Colorimetric)
Folate
Tetrahydrofolate
Tetrahydrofolic acid
THFA
Background
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated FA. The competitive inhibition reaction is launched between with HRP-conjugated FA and FA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Publications for Folic acid ELISA Kit (NBP2-59966)(1)
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Alhuthali H, Almehmadi M, Ataya E et al. Neutrophilia and its correlation with increased inflammatory response in COVID-19 in diabetic and pre-diabetic patients European Journal of Inflammation 2023-12-01 (ELISA)
ELISA
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