Folic acid Antibody (FA1)

Product Discontinued

Folic acid Antibody (FA1) Summary

• Immunogen: Derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with folic acid conjugated with BSA.
• Specificity: This recognizes free folic acid.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: Use in FLOW reported in scientific literature (PMID 25692571). This antibody is CyTOF ready.

Reactivity Notes

Chemical. Human reactivity reported in scientific literature (PMID: 25692571).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Folic acid Antibody (FA1)

• Folate
• Tetrahydrofolate
• Tetrahydrofolic acid
• THFA

Background

Folic acid and Folate (the anion form) are forms of a water-soluble B vitamin. These occur naturally in food and can also be taken as supplements. The biologically active form of folic acid is tetrahydrofolic acid (THFA), which is derived by the 2-step reduction of folate involving dihydrofolate reductase. THFA plays a key role in the transfer of 1-carbon units (such as methyl, methylene, and formyl groups) to the essential substrates involved in the synthesis of DNA, RNA, and proteins. More specifically, THFA is involved with the enzymatic reactions necessary to synthesis of purines, thymidine, and amino acids. Manifestations of folate deficiency thereafter, not surprisingly, would involve impairment of cell division, accumulation of possibly toxic metabolites such as homocysteine, and impairment of methylation reactions involved in the regulation of gene expression, thus increasing neoplastic risks. Folate is necessary for the production and maintenance of new cells. This is especially important during periods of rapid cell division and growth such as infancy and pregnancy when a deficiency can result in neural tube defects. In folate deficiency the bone marrow is affected as it is a site of rapid cell turnover. Large red blood cells called megaloblasts are produced, resulting in megaloblastic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Folic acid Antibody (NB100-72975)(2)

Publications using NB100-72975

• Zhang B, Wang Y, Huang S et al. Photoswitchable CAR-T Cell Function In Vitro and In Vivo via a Cleavable Mediator Cell Chem Biol 2020-10-20 [PMID: 33113407]
• Kim MS, Ma JS, Yun H et al. Redirection of Genetically Engineered CAR-T Cells Using Bifunctional Small Molecules J. Am. Chem. Soc. 2015-02-24 [PMID: 25692571] (FLOW, Human)

FAQs for Folic acid Antibody (NB100-72975). (Showing 1 - 1 of 1 FAQs).

1. What is the concentrations of the current lot of NB100-72975?
   • For this item, our current lot (12/12-FA3-A1) is provided at 7.0mg/ml. The concentrations for this product typically range between 2-8mg/ml.