Fluoromount

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Product Details

Summary
Applications ICC/IF, IHC, IHC-Fr, IHC-P

Order Details

Fluoromount Summary

Specificity
Aqueous mounting medium for fluorescent staining

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
Application Notes

Mounting: 1. Remove the red tip from the bottle and cut the tip off with a sharp razor or a scissors. This will help smooth flow of Fluoromount and will prevent formation of tiny bubbles because of squeezing.

2. Place the bottle upside down in a container before using Fluoromount. This will help clear Fluoromount of tiny bubbles.

3. Blot excess water from the glass without letting tissue specimens to become dry. Make sure that tissue should be wet prior to mounting.

4. Apply 2-3 drops of Fluoromount on the tissue sections and gently apply a cover slip. Allow Fluoromount to dry for 30-45 min at room temperature. Seal the edges of the coverslip with nail polish for long term storage.



Removal of Fluoromount: Fluoromount is an aqueous based mounting medium and can easily be removed by soaking the slides in deionized water. Place the slide in a beaker full of deionized water on a magnetic stirrer. Leave the slide for few hours to overnight with gentle stirring for complete removal of dry Fluoromount.
Reviewed Applications
Read 1 Review rated 5
using
NB900-66726 in the following application:

Publications
Read Publications using
NB900-66726 in the following applications:

Packaging, Storage & Formulations

Storage
Store at room temperature.
Preservative
Sodium Azide

Background

Fluoromount is an aqueous based mounting medium for cover slipping tissues stained with various fluorescent dyes. This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. However, it can be used with other fluorescent markers such as Texas Red and rhodamine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Fluoromount (NB900-66726)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC, IHC-P.


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FLOW
(1)
ICC/IF
(1)
IHC
(4)
IHC-P
(2)
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Human
(4)
Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB900-66726 Applications Species
Satyanarayanan SK, Shih YH, Chien YC, Huang SY. Anti-Oxidative Effects of Melatonin Receptor Agonist and Omega-3 Polyunsaturated Fatty Acids in Neuronal SH-SY5Y Cells: Deciphering Synergic Effects on Anti-Depressant Mechanisms. Mol. Neurobiol. 2018 Feb 03 [PMID: 29397559] (IHC, Human) IHC Human
Sirbulescu RF, Boehm CK, Soon E et al. Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions Wound Repair Regen 2017 Sep 18 [PMID: 28922523] (IHC) IHC
England C, Jiang D, Hernandez R et al. ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer. Mol Pharm 2017 Sep 06 [PMID: 28825843] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
England C, Jiang D, Ehlerding E et al. 89Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer. Eur J Nucl Med Mol Imaging 2017 Aug 19 [PMID: 28821924] (IHC, Human) IHC Human
Das V, Kroin JS, Moric M, Buvanendran A. Biochemical and Pharmacological Characterization of a Mice Model of Complex Regional Pain Syndrome. Reg Anesth Pain Med 2017 Jun 13 [PMID: 28609318] (IHC-P, Human) IHC-P Human
Eckstein M, Vaeth M, Fornai C et al. Store-operated Ca(2+) entry controls ameloblast cell function and enamel development. JCI Insight. Mar 23 2017 [PMID: 28352661] (IHC, Mouse) IHC Mouse
Rotondi S, Modarelli A, Oliva MA et al. Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells. Mol. Cell. Endocrinol. 2016 Feb 10 [PMID: 26872613] (IHC-P) IHC-P

Review for Fluoromount (NB900-66726) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NB900-66726:
Filter by Applications
ICC/IF
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence Fluoromount NB900-66726
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reviewed by:
Christopher England
ICC/IF 12/05/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotE709-C

Comments

CommentsWe add a small drop of the solution onto the slide before adding the coverslip.

Product General Protocols

View specific protocols for Fluoromount (NB900-66726): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Fluoromount (NB900-66726). (Showing 1 - 2 of 2 FAQs).

  1. Will NB900-66726 solidify after application?
    • NB900-66726 is an aqueous mounting, and it won't really harden. For long-term storage, you should seal the edges of the cover slip with clear nail polish.
  2. Does NB900-66726 contains glycerol?
    • No, Fluoromount does not contain any glycerol.

Additional Fluoromount Products

Fluoromount NB900-66726

Blogs on Fluoromount

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Contact Information

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Recent Reviews

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Christopher England
12/05/2016
Application: ICC/IF
Species: