Mounting: 1. Remove the red tip from the bottle and cut the tip off with a sharp razor or a scissors. This will help smooth flow of Fluoromount and will prevent formation of tiny bubbles because of squeezing.
2. Place the bottle upside down in a container before using Fluoromount. This will help clear Fluoromount of tiny bubbles.
3. Blot excess water from the glass without letting tissue specimens to become dry. Make sure that tissue should be wet prior to mounting.
4. Apply 2-3 drops of Fluoromount on the tissue sections and gently apply a cover slip. Allow Fluoromount to dry for 30-45 min at room temperature. Seal the edges of the coverslip with nail polish for long term storage.
Removal of Fluoromount: Fluoromount is an aqueous based mounting medium and can easily be removed by soaking the slides in deionized water. Place the slide in a beaker full of deionized water on a magnetic stirrer. Leave the slide for few hours to overnight with gentle stirring for complete removal of dry Fluoromount.
Fluoromount is an aqueous based mounting medium for cover slipping tissues stained with various fluorescent dyes. This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. However, it can be used with other fluorescent markers such as Texas Red and rhodamine.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for Fluoromount (NB900-66726)(1)
We have publications tested in 1 application: IHC-P.