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Product Details

Applications ICC/IF, IHC

Order Details

Fluoromount Summary

Aqueous mounting medium for fluorescent staining


  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. It can also be used with other fluorescent markers such as Texas Red and rhodamine.

Protocol Recommendations
1. Mounting Procedure: Remove red tip from the bottle and cut thetip off with a sharp razor or scissors. This will help achieve asmooth flow of Fluoromount and prevent formation of tinybubbles.
2. Place the bottle upside down in a container before use. This will also help clear Fluoromount of bubbles.
3. Blot excess water from the slide without letting tissue specimendry. Make sure tissue is wet prior to mounting.
4. Apply 2-3 drops of Fluoromount on the tissue section. Gentlyrotate the slide to make a thin. Make sure the tissue is completely covered.
5. Allow Fluoromount to dry for 30-45 minutes at room temperature.
6. Seal coverslip edges with nail polish for long term storage.
7. Removal of Fluoromount: Fluoromount is an aqueous-based mounting medium that can easily be removed by soaking theslides in de-ionized water.
8. Place the slide in a beaker full of de-ionized water on a magnetic stirrer.
9. Leave the slide for few hours or overnight with gentle stirring for complete removal of dry Fluoromount.
Reviewed Applications
Read 1 Review rated 5
NB900-66726 in the following applications:

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NB900-66726 in the following applications:

Packaging, Storage & Formulations

Store at room temperature.
0.05% Sodium Azide


Fluoromount is an aqueous based mounting medium for cover slipping tissues stained with various fluorescent dyes. This product is compatible with FITC, phycoerythrin, phycocyanin and allophycocyanin. However, it can be used with other fluorescent markers such as Texas Red and rhodamine.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Fluoromount (NB900-66726)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IF/IHC, IHC-P.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB900-66726 Applications Species
Costiniti V, Bomfim GH, Li Y et al. Mitochondrial Function in Enamel Development Front Physiol 2020-05-29 [PMID: 32547417] (ICC/IF) ICC/IF
Ching JY, Huang BJ, Hsu YT, Khung YL Anti-Adhesion Behavior from Ring-Strain Amine Cyclic Monolayers Grafted on Silicon (111) Surfaces Sci Rep 2020-05-29 [PMID: 32472042] (ICC/IF) ICC/IF
Wilson KD, Ochoa LF, Solomon OD et al. Elimination of intravascular thrombi prevents early mortality and reduces gliosis in hyper-inflammatory experimental cerebral malaria J Neuroinflammation 2018-06-04 [PMID: 29866139] (IF/IHC) IF/IHC
Kang Lei, Jiang Dawei, Ehlerding Emily B et al. Noninvasive Trafficking of Brentuximab Vedotin and PET Imaging of CD30 in Lung Cancer Murine Models. Molecular Pharmaceutics 2018-03-20 [PMID: 29537283] (IF/IHC) IF/IHC
Satyanarayanan SK, Shih YH, Chien YC, Huang SY. Anti-Oxidative Effects of Melatonin Receptor Agonist and Omega-3 Polyunsaturated Fatty Acids in Neuronal SH-SY5Y Cells: Deciphering Synergic Effects on Anti-Depressant Mechanisms. Mol. Neurobiol. 2018-02-03 [PMID: 29397559] (IF/IHC, Human) IF/IHC Human
Sirbulescu RF, Boehm CK, Soon E et al. Mature B cells accelerate wound healing after acute and chronic diabetic skin lesions Wound Repair Regen 2017-09-18 [PMID: 28922523] (IF/IHC) IF/IHC
England CG, Jiang D, Hernandez R et al. ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer Mol Pharm 2017-08-22 [PMID: 28825843] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
England CG, Jiang D, Ehlerding EB et al. (89)Zr-labeled nivolumab for imaging of T-cell infiltration in a humanized murine model of lung cancer Eur J Nucl Med Mol Imaging 2017-08-20 [PMID: 28821924] (IF/IHC, Mouse) IF/IHC Mouse
Das V, Kroin JS, Moric M, Buvanendran A. Biochemical and Pharmacological Characterization of a Mice Model of Complex Regional Pain Syndrome. Reg Anesth Pain Med 2017-06-13 [PMID: 28609318] (IHC-P, Human) IHC-P Human
Eckstein M, Vaeth M, Fornai C et al. Store-operated Ca(2+) entry controls ameloblast cell function and enamel development. JCI Insight. 2017-03-23 [PMID: 28352661] (IF/IHC, Mouse) IF/IHC Mouse
Show All 13 Publications.

Review for Fluoromount (NB900-66726) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NB900-66726:
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Images Ratings Applications Species Date Details
 Fluoromount NB900-66726
reviewed by:
Christopher England




CommentsWe add a small drop of the solution onto the slide before adding the coverslip.

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for Fluoromount (NB900-66726). (Showing 1 - 2 of 2 FAQs).

  1. Will NB900-66726 solidify after application?
    • NB900-66726 is an aqueous mounting, and it won't really harden. For long-term storage, you should seal the edges of the cover slip with clear nail polish.
  2. Does NB900-66726 contains glycerol?
    • No, Fluoromount does not contain any glycerol.

Additional Fluoromount Products

Array NB900-66726

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Christopher England