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| Application Notes | FluorEver is a mounting medium which significantly extends a light-emitting capacity of fluorescent dyes which tend to fade during storage and microscopy examination. FluorEver can be used for various applications including, but not limited to, fluorescence imunohistochemistry and cytochemistry, fluorescence neuronal tracing, and gene expression studies (using, for example, GFP and YFP tags). FluorEver medium is compatible with cryostat and paraffin-embedded tissue sections, as well as with cultured neuronal and non-neuronal cells. Unlike other types of mounting media, FluorEver protects cells and tissues from shrinking thus maintaining their native cytological and histological architecture, which is of critical importance for accurate image analysis studies FluorEver blocks fading of a large variety of fluorescent dyes within a broad range of excitation and emission spectra, which makes this mounting medium a reagent of choice for maintaining brightness of multi-color fluorescence samples during storage and analysis by either conventional fluorescence or laser confocal microscopy. Use in ICC/IF reported in secitific publication PMID: 32371496 |
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| Storage | Store at -20C in the dark. Avoid freeze-thaw cycles. |
| Publication using NBP2-60229 | Applications | Species |
|---|---|---|
| Duron DI, Lei W, Barker NK et al. Inhibition of Hsp90 in the spinal cord enhances the antinociceptive effects of morphine by activating an ERK-RSK pathway Sci Signal 2020-05-05 [PMID: 32371496] (ICC/IF) | ICC/IF |