Fluorescent Exosome Standards (B16F10 cell line)

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Fluorescent Exosome Standards (B16F10 cell line) [NBP3-11689] - Absorption and corrected fluorescence emission spectrum of conjugate excitation at 488 nm. Excitation spectrum (dotted line) and emission spectrum (solid ...read more

Product Details

Summary
Applications EM, Flow
Format
Fluorescent

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Fluorescent Exosome Standards (B16F10 cell line) Summary

Description
Highly pure fluorescent exosome standards with superior performance, suitable for extracellular vesicle (EV) tracking studies, flow cytometry, and electron microscopy.
Preparation
Method
Exosome isolation involves a combination of ultracentrifugation and microfiltration procedures. Fluorescent exosomes are subsequently quantified and validated for overall protein content and particle number by Nanoparticles Tracking Analysis.

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • Flow Cytometry
Application Notes
The excitation maximum of fluorescent exosome standards is 500 nm - 650 nm and emission maximum is 510 - 665 nm.

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
Cell culture media

Notes

One vial contains 100 ug of purified exosomes (measured as total protein content; number of particles in 100 ug: > 1x10^10). Fluorescent labeled exosomes are stable for approximately 6 months storage at -20C. Avoid repeated freeze-and-thaw cycles. Protect from light

Background

Exosomes are small endosome derived lipid nanoparticles (30-150nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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