![Functional: Recombinant Human, Mouse Flagellin TLR5 Mutant His Protein [NBP3-18159] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.) activate TLR5 in vivo.Method: Mice WT are injected i.v. with 1ug of Flagellin (rec.) (=WT) or Flagellin (NLRC4 Mutant) (rec.). After 2 hours, levels of CCL20 in the serum are measured by ELISA. As control, WT mice are injected i.v. with 1ug of Flagellin (TLR5 Mutant) (rec.). PBS injected mice are used as negative control.](http://images.novusbio.com/fullsize/Recombinant-Human--Mouse-Flagellin-TLR5-Mutant-His-Protein-Functional-NBP3-18159-img0001.jpg "Functional: Recombinant Human, Mouse Flagellin TLR5 Mutant His Protein [NBP3-18159] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.) activate TLR5 in vivo.Method: Mice WT are injected i.v. with 1ug of Flagellin (rec.) (=WT) or Flagellin (NLRC4 Mutant) (rec.). After 2 hours, levels of CCL20 in the serum are measured by ELISA. As control, WT mice are injected i.v. with 1ug of Flagellin (TLR5 Mutant) (rec.). PBS injected mice are used as negative control.")
| Reactivity | BaSpecies Glossary |
| Applications | Bioactivity |
| Concentration | LYOPH |
| Description | Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a substitution of amino acids 90-97 required for TLR5 signaling by a motif from a non-signaling flagellin and fused at the N-terminus to a His-tag. Source: E. coli NCBI: NP_460912 |
| Specificity | Not detected by human and mouse TLR5 (Toll-like receptor 5). |
| Details of Functionality | Not detected by human and mouse TLR5 (Toll-like receptor 5). Does not activate TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. It can be used as negative control for TLR5 signaling assays. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Purity | >95% pure by SDS-PAGE |
| Endotoxin Note | <0.01EU/ug purified protein (LAL test; Lonza). |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95% pure by SDS-PAGE |
| Reconstitution Instructions | Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution) |
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