Recombinant Bacteria Flagellin NLRC4 Mutant His Protein

Functional: Recombinant Human, Mouse Flagellin NLRC4 Mutant His Protein [NBP3-18158] - Flagellin (rec.) and Flagellin (NLRC4 Mutant) (rec.)induce CCL20-luciferase promoter through TLR5 in vitro.Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid are activated for 24h with the indicated concentrations of Flagellin (rec.)and (NLRC4 Mutant) (rec.). As a negative control, Flagellin (TLR5 Mutant) (rec.) is used. Luciferase assays are performed on cell lysates and luminescence is normalized to mock-treated cells.

• Reactivity: Ba
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Bacteria Flagellin NLRC4 Mutant His Protein Summary

• Description: Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a small deletion at the C-terminus of the protein required for NLRC4 activation and fused at the N-terminus to a His-tag.
  
  Source: E. coli

  NCBI: NP_460912

• Specificity: Detected by human and mouse TLR5 (Toll-like receptor 5). Not detected by mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).
• Details of Functionality: Detected by human & mouse TLR5. Not detected by mouse NLRC4. Activation of TLR5 in human epithelial cell assays based on NF-kB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at 5-50ng/ml & in vivo at a starting concentration of 0.1-1 ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages (in the presence of streptolysin O (SLO)). It can be used as negative control for NLRC4 signaling.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: <0.01EU/ug purified protein (LAL test; Lonza).

Applications/Dilutions

• Dilutions:
  • Bioactivity

Reactivity Notes

Salmonella enterica Serovar Typhimurium strain (ATCC14028). Cross-reactive with Human, Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95% pure by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 100 ul sterile water (0.1mg/ml after reconstitution)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Uniprot:
  • NP_460912(Bacteria)