FK506 ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA, Quantification
Suitable Sample Type
Plasma, Serum
Sensitivity
The actual detection limit is based on the setup of experiment protocol, such as extraction procedure and dilution factor.

Order Details

FK506 ELISA Kit (Colorimetric) Summary

Description
FK-506 ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of FK-506.
Sensitivity
The actual detection limit is based on the setup of experiment protocol, such as extraction procedure and dilution factor.
Assay Type
Competitive ELISA
Sample Volume
25 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
Useful for quantification.
Reviewed Applications
Read 2 Reviews rated 4.5
using
KA1417 in the following application:

Publications
Read Publication using KA1417.

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Wash Buffer (10x PBS-Tween). Dilute 10 fold with distilled or deionized water to150 mL prior to use (#6).
  2. Stop Solution, 3 N HCl (#7).
  3. Stabilized Tetramethylbenzidine (TMB) substrate. Ready to use (#5).
  4. FK-506-Horseradish Peroxidase conjugate (ACC-HRP) (#3).
  5. Calibrator: Containing 0, 2, 10 and 50 ng/mL of FK-506.
  6. 96-wells microtiter plate: Twelve strips of 8 detachable wells, coated withAnti-FK-506 antibody (#S).

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for FK506 ELISA Kit (KA1417)(1)

Reviews for FK506 ELISA Kit (KA1417) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using KA1417:
Filter by Applications
ELISA
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
ELISA FK506 KA1417
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reviewed by:
Xinxin Feng
ELISA Human 07/02/2015
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Summary

ApplicationELISA
Sample Testedstandard Tac solutions
SpeciesHuman

Blocking

Blocking Detailscompetitive assay so this is not applicable

Primary Anitbody

Dilution Ratioprovided

Details

Detection NotesUV 450nm with a plate reader
ELISA Plate Detailsprovided with the kit
Wash Descriptionwash with washing buffer provided for 3 times (~360uL each wash)

Comments

Commentsprotocol1. add 25uL std, mix by lightly tilting 2. add 100uL Tac-HRP, mix by lightly tilting, incubate RT 30min3. invert the strip and empty the solution, wash with washing buffer for 3 times (~360uL each wash)4. add 100uL TMB, mix by lightly tilting, incubate RT 15 min5. add 100uL stop solution, mix by lightly tilting, read at 450nm
ELISA FK506 KA1417
Enlarge
reviewed by:
Anonymous
ELISA Mouse 03/01/2014
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Summary

ApplicationELISA
Sample TestedMouse serum
SpeciesMouse
CommentsThis is a foolproof protocol, and one of the shortest and easiest ELISAs I have done. Kit performed reliably across samples, with minimal variations likely reflective of injection technique and drug dose delivered. I do note, however, amount of non-specific staining, where background fluorescence can be averaged and subtracted from raw data to achieve expected results.

Comments

CommentsThis is a foolproof protocol, and one of the shortest and easiest ELISAs I have done. Kit performed reliably across samples, with minimal variations likely reflective of injection technique and drug dose delivered. I do note, however, amount of non-specific staining, where background fluorescence can be averaged and subtracted from raw data to achieve expected results.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FK506 ELISA Kit (KA1417). (Showing 1 - 1 of 1 FAQ).

  1. Has use of this kit been published? What is the sensitivity of FK-506 detection? Thank you.
    • Our FK506 ELISA kit with catalogue number KA1417 is manufactured by Abnova, a Taiwanese company for whom we distribute. We are not currently aware of any publications which cite the use of this product. Please find here a link to Abnova's product page for KA1417. I have now heard back from Abnova, who informed me that the detection range of their FK506 ELISA kit, KA1417, is between 1-60ng/ml.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Xinxin Feng
07/02/2015
Application: ELISA
Species: Human

Anonymous
03/01/2014
Application: ELISA
Species: Mouse