FIV gp95 Antibody (SU1-30) - BSA Free

• Reactivity: V-Vi
• Applications: WB, Flow
• Clone: SU1-30
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FIV gp95 Antibody (SU1-30) - BSA Free Summary

• Immunogen: Made to FIV gp95
• Specificity: NB100-64378 specifically recognizes the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU,gp95. The primary receptor for FIV infection of cats has been identified as the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion in the CD4+ population. Studies have shown that the V3 loop of SU,gp95 is a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU,gp95 to CXCR4.(3) Clone SU1-30 has been shown to inhibit the sCD134-activated entry of both clade B and clade C FIV strains.(1) NB100-64378 does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of Sodium Azide is recommended prior to use in functional studies.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000
• Application Notes: Detects a band of approximately 95kDa under reducing conditions.

Reactivity Notes

Reacts with Viral.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Background

The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for FIV gp95 Antibody (NB100-64378). (Showing 1 - 1 of 1 FAQ).

1. Is there anything known concerning the use of this clone in IF yet?
   • NB100-64378 has not yet been tested in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.