Reactivity | V-ViSpecies Glossary |
Applications | WB, ELISA, Flow, ICC/IF |
Clone | CE4-13B1 (13B1) |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1.0 mg/ml |
Immunogen | Purified gp95 envelope antigen. |
Specificity | NB100-66553 is specific for FIV Envelope gp95 protein. This antibody is directed against an epitope within the variable V2 region and cross-reacts with Petaluma, 34TF10, Ppr, MCH5-5 and Glasgow 8 isolates. NB100-66553 does not neutralize FIV and does not recognize Feline Leukaemia Virus, Feline Herpes Virus Type 1, Feline Coronavirus and Feline Calicivirus. |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Mouse |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
|
Application Notes | Western Blot, Immunofluorescence, ELISA and Flow Cytometry In WB using ultra-centrifuged FIV under reducing conditions, this antibody identifies a major band at approximately 95kD, and another at approx. 25kD which is a degradation product of gp95 and is not the gag 24 peptide. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS (pH 7.2) |
Preservative | 0.05% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.