FIV Envelope Antibody (CE4-13B1 (13B1))

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
CE4-13B1 (13B1)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FIV Envelope Antibody (CE4-13B1 (13B1)) Summary

Immunogen
Purified gp95 envelope antigen.
Specificity
NB100-66553 is specific for FIV Envelope gp95 protein. This antibody is directed against an epitope within the variable V2 region and cross-reacts with Petaluma, 34TF10, Ppr, MCH5-5 and Glasgow 8 isolates. NB100-66553 does not neutralize FIV and does not recognize Feline Leukaemia Virus, Feline Herpes Virus Type 1, Feline Coronavirus and Feline Calicivirus.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Western Blot, Immunofluorescence, ELISA and Flow Cytometry In WB using ultra-centrifuged FIV under reducing conditions, this antibody identifies a major band at approximately 95kD, and another at approx. 25kD which is a degradation product of gp95 and is not the gag 24 peptide.

Reactivity Notes

Viral.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Background

The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FIV Envelope Antibody (NB100-66553) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-66553AF350
Alexa Fluor 405 NB100-66553AF405
Alexa Fluor 488 NB100-66553AF488
Alexa Fluor 532 NB100-66553AF532
Alexa Fluor 594 NB100-66553AF594
Alexa Fluor 647 NB100-66553AF647
Alexa Fluor 700 NB100-66553AF700
Alexa Fluor 750 NB100-66553AF750
Allophycocyanin NB100-66553APC
Allophycocyanin/Cy7 NB100-66553APCCY7
DyLight 350 NB100-66553UV
DyLight 405 NB100-66553V
DyLight 488 NB100-66553G
DyLight 550 NB100-66553R
DyLight 594 NB100-66553DL594
DyLight 650 NB100-66553C
DyLight 680 NB100-66553FR
DyLight 755 NB100-66553IR
Janelia Fluor 549 NB100-66553JF549
Janelia Fluor 646 NB100-66553JF646
PE NB100-66553PE
PE/Atto594 NB100-66553PEATT594
PE/Cy5.5 NB100-66553PECY55
PE/Cy7 NB100-66553PECY7
PerCP NB100-66553PCP

Additional FIV Envelope Products

Array NB100-66553

Blogs on FIV Envelope

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Contact Information

Learn the difference between western blot and simple western

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