Filamentous Hemagglutinin Antibody (352-02)

Product Discontinued

Filamentous Hemagglutinin Antibody (352-02) Summary

• Immunogen: Whole cell pertussis vaccine + FHA.
• Specificity: FHA from Bordetella pertussis.Cross-reactivity to cerebral microvessels has been reported.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: fhaB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Background

Filamentous hemagglutinin (FHA) of Bordetella pertussis is a 220 kDa large adhesive surface protein which together with the pertussis toxin (PTx) facilitates attachment of the bacteria to human ciliated cells and macrophages during the course of whooping cough. FHA can recognize and bind the leukocyte-restricted adhesion molecule (CD11b/CD18, Mac-1) suggesting that FHA mimics a natural ligand on endothelial cells for this integrin which enables FHA to competitively interfere with CD18-dependent leukocyte migration to the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: fhaB