Mouse Eye Tissue Lysate (Normal)

• Reactivity: Mu
• Applications: WB
• Concentration: 2 mg/ml

Mouse Eye Tissue Lysate (Normal) Summary

• Specificity: Mouse whole eye tissue from balb/c mouse.

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
• Buffer: SDS sample buffer containing 50 mM DTT.
• Concentration: 2 mg/ml

Lysate Details for Array

• Type: Tissue
• Tissue: Visual
• Tissue Condition: Normal

Background

Eye tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.