Human Eye Tissue Lysate (Normal)

Product Discontinued

Human Eye Tissue Lysate (Normal) Summary

• Specificity: Human whole eye tissue.

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied in SDS sample buffer containing 5% beta-mercaptoethanol.
• Concentration: 2 mg/ml

Lysate Details for Array

• Type: Tissue
• Tissue: Visual
• Tissue Condition: Normal

Background

Whole Eye tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl_2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.