Human Exosome (Urine) ELISA Kit (Colorimetric)

ELISA: Human Exosome (Urine) ELISA Kit (Colorimetric) [NBP3-14800] - Example of standard curves: CD9 Titration of COLO1 (1) and plasma (2) Exosome Standards. This standard curve is for demonstration only. For quantification purposes a standard curve must be obtained with every assay.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Human Urine
• Sensitivity: < 0.35 ug of overall exosome or < 50 pg targeted exosomes protein

Human Exosome (Urine) ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit ensures dose-dependent exosome detection in a variety of samples.
  
  The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.
  
  Up to 100 uL of biological sample can be loaded per well.
  
  Concentrated urine samples are recommended prior to capture.
  
  Expiration date is 12 months. Stored up to 24 months (if unopened). After opening, buffers and solutions are good for 1 month, plate strips are good for 6 months. After reconstitution, standards can be stored at -20C for 1 month, -80C for 6 months. Avoid Freeze-thaw cycles.
• Sensitivity: < 0.35 ug of overall exosome or < 50 pg targeted exosomes protein
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Exosome (Urine) ELISA Kit (Colorimetric)

• EV detection
• exosome detection
• extracellular vesicle detection

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and have an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Exosome (Urine) ELISA Kit (NBP3-14800). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.