Human Exosome (Urine) ELISA Kit (Colorimetric)

Overall Exosome Capture and Quantification (Urine, Colorimetric) Kit [NBP2-49783] - Principle underlying the immunocapture and quantification of the exosomes with this kit

Product Discontinued

Human Exosome (Urine) ELISA Kit (Colorimetric) Summary

• Description: Features
  
• This ELISA kit ensures dose-dependent exosome detection in a variety of samples.
• The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample
• Exosome comprehensive profiling
• Up to 100 µl of biological sample can be loaded per well
• Concentrated urine samples are recommended prior to capture.
• Specificity: Human urine exosomes
• Sensitivity: 39 pg
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 13
• Intra-Assay: CV% < 10
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Human Exosome (Urine) ELISA Kit (Colorimetric)

• EV detection
• exosome detection
• extracellular vesicle detection

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and have an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.