Human Exosome (Serum) ELISA Kit (Colorimetric)

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ELISA: Human Exosome (Serum) ELISA Kit (Colorimetric) [NBP3-11771] - Example of standard curves: CD9 Titration of COLO1 (1) and plasma (2) Exosome Standards. This standard curve is for demonstration only. For ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
human serum
Sensitivity
< 0.35 ug of overall exosome or < 50 pg targeted exosomes protein

Order Details

Human Exosome (Serum) ELISA Kit (Colorimetric) Summary

Sensitivity
< 0.35 ug of overall exosome or < 50 pg targeted exosomes protein
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoassay

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Exosome standards (healthy human serum)
  2. Pre-coated ELISA plate (pan exosome)
  3. Primary detection antibody (anti-CD9, Mouse mAb)
  4. Secondary antibodies (anti-mouse-HRP)
  5. Stop solution
  6. Substrate solution

Alternate Names for Human Exosome (Serum) ELISA Kit (Colorimetric)

  • EV detection
  • exosome detection
  • extracellular vesicle detection

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Exosome (Serum) ELISA Kit (NBP3-11771). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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