Human Exosome (Serum) ELISA Kit (Colorimetric)

Overall Exosome Capture and Quantification (Serum, Colorimetric) Kit [NBP2-49785] - Basic principle for the immunocapture and quantification of exosomes with this kit

Product Discontinued

Human Exosome (Serum) ELISA Kit (Colorimetric) Summary

• Description: Features
  
• Ensures dose-dependent exosome detection in a variety of samples.
• Quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample
• Exosome comprehensive profiling
• Up to 100 µl of biological sample can be loaded per well
• Unfractionated serum sample can be directly used for capture
• Assay Type: Sandwich ELISA
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay
• Application Notes: The kit contains lyophilized exosome standards for assay calibration.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Alternate Names for Human Exosome (Serum) ELISA Kit (Colorimetric)

• EV detection
• exosome detection
• extracellular vesicle detection

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.Novus kit is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. It consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Exosome (Serum) ELISA Kit (NBP2-49785)(1)

Publication using NBP2-49785

• Jiang L, Cao H, Deng T et al. Serum exosomal miR-377-3p inhibits retinal pigment epithelium proliferation and offers a biomarker for diabetic macular edema The Journal of international medical research 2021-04-01 [PMID: 33906524] (ELISA, Human)

FAQs for Exosome (Serum) ELISA Kit (NBP2-49785). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.