Human Exosome (Cell Media) ELISA Kit (Colorimetric)

ELISA: Human Exosome (Cell Media) ELISA Kit (Colorimetric) [NBP3-11772] - Example of standard curves: CD9 Titration of COLO1 (1) and plasma (2) Exosome Standards. This standard curve is for demonstration only. For quantification purposes a standard curve must be obtained with every assay.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant (pre-concentrated)
• Sensitivity: < 0.35 ug of overall exosome or < 50 pg targeted exosomes protein

Human Exosome (Cell Media) ELISA Kit (Colorimetric) Summary

• Sensitivity: < 0.35 ug of overall exosome or < 50 pg targeted exosomes protein
• Assay Type: Sandwich ELISA
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Exosome (Cell Media) ELISA Kit (Colorimetric)

• EV detection
• exosome detection
• extracellular vesicle detection

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Exosome (Cell Media) ELISA Kit (NBP3-11772)(1)

Publication using NBP3-11772

• Liu S, Gong J miR-124-3p Delivered Using Exosomes Attenuates the Keratinocyte Response to IL-17A Stimulation in Psoriasis Oxidative medicine and cellular longevity 2022-10-12 [PMID: 36275890] (ELISA)

FAQs for Exosome (Cell Media) ELISA Kit (NBP3-11772). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.